the usefulness of anti-hcv signal to cut-off ratio in predicting viremia in anti-hcv in patients with hepatitis c virus infection

نویسندگان

fahimeh ranjbar kermani blood transfusion research center, high institute for research and education in transfusion medicine, tehran, ir iran

zohreh sharifi blood transfusion research center, high institute for research and education in transfusion medicine, tehran, ir iran; blood transfusion research center, high institute for research and education in transfusion medicine, p.o. box: 14665-1157, tehran, ir iran. tel/fax: +98-218860150130

fereshteh ferdowsian blood transfusion research center, high institute for research and education in transfusion medicine, tehran, ir iran

zahrah paz blood transfusion research center, high institute for research and education in transfusion medicine, tehran, ir iran

چکیده

objectives the current study aimed to assess relationship between s/co rates and hcv-rna levels in the laboratory to identify hcv viremia in patients with a positive anti-hcv. patients and methods all serum samples were assayed for anti-hcv by elisa method. a total of 265 anti-hcv positive patients were tested for hcv-rna testing by quantitative method using artus hcv rg real-time polymerase chain reaction (rt- pcr) kit. statistical analysis was done by spss version 16. results of the 265 patients with hcv infection, 204 (77%) were male and the mean age was 43.53 ± 13.17 years, ranging 1 - 81 years. no correlation was found between s/co ratios and hcv-rna levels. there was significant difference in s/co ratio between viremic and non-viremic subjects. the sensitivity, specificity, negative predictive value, and positive predictive value were 100%, 81.4%, 100%, and 77.2%, respectively in the s/co ratio of 2.7. background hepatitis c virus (hcv) infection is diagnosed by antibody and rna based methods. patients with anti-hcv sample rate/cutoff rate (s/co) ratios > 1 are reported as anti-hcv positive. rna based methods are introduced to confirm positivity in seropositive samples. conclusions the present study indicated that anti-hcv s/co ratio is useful to predict non-viremic patients. a cut-off value of 2.7 can determine the usefulness of hcv-rna testing. patients with s/co < 2.7 are not viremic; therefore, hcv-rna testing is not recommended. it is suggested that laboratories report s/co ratio along with anti-hcv results to manage hcv infection better, especially in countries that quantitative hcv testing is expensive or not available.

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عنوان ژورنال:
jundishapur journal of microbiology

جلد ۸، شماره ۴، صفحات ۰-۰

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